This is out new brine shrimp hatchery! We stared off putting in 500mL of pure water(must be pure water) then with about a 2% salt concentration which is needed for brine shrimp survival. We also hooked up a air pump at the bottom of the tank to allow circulation through the tank. Which is nessary for them to breathe once they hatch! They will stay in here for about 2-4 days until they are fully hatched and then they will be moved to a 5 gallon tank to grow into adults!